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KMID : 0614020060210010030
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Journal of Pharmaceutical Sciences (C.N.U.)
2006 Volume.21 No. 1 p.30 ~ p.36
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Evaluation of antioxidant activity of taurine and hypotaurine using the total oxy-radical scavenging capacity assay
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Oh Jung-Min
Zhu Hong-Mei
Kim Sang-Kyum
Kim Bong-Hee
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Abstract
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Taurine, a sulfur containing non-amino acid, is one of the most abundant intracellular amino acid in humans, and hypotaurine is a precursor of the synthesis of taurine. While it is widely believed that taurine may play an important role in protecting cells against toxic injury by functioning as an antioxidant, there is a lack of evidence to support this hypothesis. In this study, total oxy-radical scavenging capacity(TOSC) assay was used to evaluate antioxidant activity of taurine and hypotaurine against peroxyl radical, hydroxyl radical and peroxynitrite. The present results show that taurine does not have oxy-radical scavenging activity and hypotaurine is comparable to glutathione, an antioxidant substance, in oxy-radical scavenging activity. The results suggest that protective effects of taurine against oxidative stress may not be associated with its direct oxy-radical scavenging activity. Difference in oxy-radical scavenging capacity between taurine and hypotaurine may be ascribed to their sulfur oxidation state.
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KEYWORD
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taurine, hypotaurine, antioxidant, TOSC assay
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